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Apollo Hospital Apollo Hospital

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Max Hospital Max Hospital

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Asian Heart Institute
Asian Heart Institute

Wockhardt Hospital
Wockhardt Hospital

Hiranandani Hospital
Hiranandani Hospital

Sir Ganga Ram Hospital
Sir Ganga Ram Hospital

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Lilavati Hospital Lilavati Hospital

Kokilaben Hospital
Kokilaben Hospital

Narayana Hrudayalaya
Narayana Hrudayalaya

Global Hospitals Global Hospitals

Sperm FISH

Sperm FISH Routine analysis of sperm chromosomes is not feasible in clinical practice. However, interphase FISH on spermatozoa offers an accurate and reliable method of analysis even in the presence of a low sperm count. The percentage of sperm with hypo/hyperhaploidy is calculated to counsel the couple regarding chances of a normal pregnancy.

Sample : Fresh semen sample.

Outstation samples are accepted after partial processing in any andrology laboratory. The ‘swim-up’ sperm and pellet are to be separated in 2 tubes and incubated in hypotonic KCl (0.075M) for 20 minutes, following which a few drops of Carnoys fixative are added. After centrifugation, fresh fixative is added to the pellets before transportation.


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